Peroxide dismutase (POD):

The peroxidase activity was determined by Chance and Maehly (1954). The test tubes were added with 2.7ml of phosphate buffer, 0.1ml of guaiacol, and 0.1ml of enzyme extract and mixed well. The absorbance was taken at 470nm for 120 seconds, every 20 seconds. A change in absorbance of 0.01 units per minute defined one unit of POD activity. The unit used to express the specific enzyme activity was enzyme/mg protein.

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