PPSC Solved Biology Paper 2017:

1. Asmara is the Capita1 of Eritrea. 2. Great barrier, the reef is present in Australia. 3. Allan Octavian Hume was a member of civil services who established the Congress Party. 4. Robin Williams was a comedian who committed suicide. 5. “Successive” synonym “Consecutive”. 6. “Latent” antonym “Obvious”. 9. SAARC members countries 8 10. UN …

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Punjab CM Usman Buzdar tests positive for Covid-19

Chief Minister Usman Buzdar of Punjab screened positive for the novel coronavirus, said his spokesman. At CM Home, he is self-isolated and has suspended all his job engagements.  In the past week, he has chaired several sessions.  Many lawmakers have been diagnosed with the virus, which has taken over 9,000 lives in Pakistan, such as PTI’s Asad Umar and Shah Mahmood Qureshi, PPP’s Bilawal Bhutto Zardari, Saeed Ghani, and Sindh CM Murad Ali Shah. Pakistan is currently facing a second coronavirus …

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Ascorbate peroxidase (APX):

The ascorbate peroxidase working (APX, EC: 1.11.1.11) will be determined from the decrease in absorbance of ascorbic at 290 nm (Asada and Chen, 1992). The enzyme activity will be assayed in 3 mL containing 50 mM phosphate buffer at pH 7, 0.5 mM ascorbic acid, and 0.5 mM H2O2. The reaction will be started by …

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Nitrate reductase (NR):

Nitrate reductase (NR; EC 1.6.6.1) activity will be performed by measuring the amount of NO2 formed in NR assay buffer (Wray and Filner, 1970). The assay medium for NR contained 0.5 ml potassium phosphate buffer (100 mM, pH 7.5), 0.1 ml KNO3 (100 mM), 0.1 ml NADH (0.15 mM) and 0.1 ml extract in a …

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