Catalase activity:

Chance and Maehly (1954) method was followed with minor changes for evaluating the catalase activity. The activity of catalase was determined by measuring the reduction in absorbance for 120 seconds, every 20 seconds at 240nm. The reaction mixture contained 2.8ml of phosphate buffer along with the addition of 0.1ml of H2O2, 0.1ml of enzyme extract … Read more

MDA contents:

MDA in the leaves was investigated by the method of Carmak & Horst (1991). This reaction was based on Thiobarbituric acid (TBA). Fresh leaves (0.5g) were ground properly in 10 ml of 0.1% tri-chloroacetic acid solution and centrifuged for 10 minutes at 12,000rpm. 1ml of the supernatant was reacted with 1 ml of 20% TCA … Read more

H2O2 contents:

Hydrogen peroxide concentration was estimated using the method of Velikova et al. (2000). 0.5g of the leaf was crushed with pestle mortar and plant material was homogenized in 10ml of 0.1% trichloroacetic acid on an ice bath. The extract was centrifuged at 12,000rpm for 10 minutes. The reaction mixture was added with 0.5ml supernatant, 0.5 … Read more

Ascorbic acid concentration:

Mukherjee and Choudhuri (1983) method will be followed for the determination of Ascorbic acid in plants. 0.5g of plant leaf was weighed by using a digital balance and added with 10ml of 6% trichloroacetic acid. The grinding was done on an ice bath. Care was taken while handling TCA as it caused skin burns. After … Read more

Total Anthocyanin:

Total anthocyanin will be determined by the method of Hodges and Nozzolillo (1996). 0.5 g of leaf material will be ground in pestle and mortar using 5ml of phosphate buffer (K2HPO4+KH2PO4, pH 7.8). Ice will be used to keep the pestle mortar cool. Centrifugation will be done at 10,000 rpm for 10 minutes while maintaining … Read more

Chlorophyll & Carotenoids Contents Protocol:

Arnon (1949) METHOD is considered while measuring the chlorophyll contents of plants along with chlorophyll a and chlorophyll b ratio and total chlorophyll contents. 0.5g of the leaf will be ground in 10ml of 80% methanol.  The extract will be centrifuged at 10,000rpm for 10 minutes. The supernatant will be stored in small bottles in … Read more

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