Catalase activity:

Chance and Maehly (1954) method was followed with minor changes for evaluating the catalase activity. The activity of catalase was determined by measuring the reduction in absorbance for 120 seconds, every 20 seconds at 240nm. The reaction mixture contained 2.8ml of phosphate buffer along with the addition of 0.1ml of H2O2, 0.1ml of enzyme extract added and was slightly shaken to mix it well. Then the absorbance was taken on the spectrophotometer.

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