Antioxidant enzymes activities:

Fresh leaves (0.5 g) will be finely ground under chilled conditions in 10 ml of phosphate buffer (50 mM with pH 7.8) for the extraction of antioxidant enzymes. Centrifugation of the mixture will be performed at 12000 × g for 20 min at 4ºC. The supernatant will be re-centrifuged at 15000 × g for 10 min and then the resultant extract stored at -20°C for determining the activity of antioxidant enzymes.

Antioxidant enzyme activities are SOD, POD, CAT, APX, GPX, and NR.

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