Relative Membrane Permeability (%):

Fresh leaves (0.5 g) will be chopped and placed in 10 ml of distilled water. Then samples will be vortexed for 5s and the electrical conductivity (ECo) determined. The test tubes will be kept at 4°C overnight and the EC1 measured. Then the samples will be autoclaved for 1 h and EC2 measured after cooling the solution to room temperature. Relative membrane permeability will be measured using the following formula:

RMP (%) = (EC1-ECo/EC2- ECo) x 100