Ascorbate peroxidase (APX):

The ascorbate peroxidase working (APX, EC: 1.11.1.11) will be determined from the decrease in absorbance of ascorbic at 290 nm (Asada and Chen, 1992). The enzyme activity will be assayed in 3 mL containing 50 mM phosphate buffer at pH 7, 0.5 mM ascorbic acid, and 0.5 mM H2O2. The reaction will be started by the addition of H2O2.

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