Total flavonoids:

Flavonoids were determined by using the AlCl3 method. 1ml of the sample was taken in cuvette and 0.3ml of NaNO2 was added. Before adding the next chemical shaken well and then added 0.3ml of AlCl3. After mixing well, 2ml of NaOH was added. The optical density was taken at 510nm via a spectrophotometer.

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